Effects of physical activity and resisted exercise on the expression of SREBF1 in C57BL/6 rats with cachexia associated with the singen tumoral model of cutaneous melanoma
Abstract
The aim of the study was to verify the effects of physical activity and resistance exercise on the expression of SREBF1 in C57BL / 6 mice with cachexia associated with the cutaneous melanoma tumor model. Twelve female C57BL / 6 mice were used, where they were randomly divided into four groups. The first group being the control (n=3), did not perform any type of intervention, the second was the control group with tumor induction (n=3), performing no intervention and being induced cachexia associated with cancer, the third group was physical activity (n=3), performing physical activity in an enriched environment and being induced by cancer-associated cachexia, the fourth group performed climbing training (n=3), performing resistance exercises with shock stimulus and cancer-induced cachexia. Before, an experiment (n=12) was carried out to define the characterization of cachexia in the singenic model of cutaneous melanoma using a murine melanoma strain B16-F10. The analysis identified an increase in the expression of SREBF1, in the control group we can identify the increase of this protein, being identified an even greater increase, when the animal presented the tumor and still performed physical activity and resisted exercises. Considering the data obtained in the present study, it is concluded that the groups with intervention after cachexia had a significant increase in SREBF1. However, the results described here cannot guide clinical actions at this moment, since another study with different intervention models in cachexia is necessary to elucidate the doubts left here.
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