The standardizing experimental process to detect stress proteins in physical exercise

  • Joaquim Ferreira Antunes Neto Laboratório de Estudos Multidisciplinares do Estresse (LEME-METROCAMP), Faculdades Integradas Metropolitanas de Campinas - METROCAMP
  • Bruna Bergo Nader Curso de graduação de Fisioterapia, Instituto de Ensino Superior de Itapira - IESI
  • Denise Vaz de Macedo Departamento de Bioquí­mica, Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP
Keywords: Scientific method, Stress proteins, Oxidative stress

Abstract

The process of standardization, reproducibility, development and analysis of results in laboratory experiments involving bench is a complex task. The possible multidisciplinarity between physical education and other areas of biological sciences had led many students for studies in basic science. However, the practices of scientific research often are not part of routine research on colleges of Physical Education. The aim of this study is to reconstruct an area instigating procedures for research –stress proteins as markers of oxidative stress –in a didactic dimension for those who want to launch new research challenges.

Author Biography

Joaquim Ferreira Antunes Neto, Laboratório de Estudos Multidisciplinares do Estresse (LEME-METROCAMP), Faculdades Integradas Metropolitanas de Campinas - METROCAMP

Doutor em Biologia Funcional em Molecular (Departamento de Bioquí­mica - IB - UNICAMP), área de Bioquí­mica

References

-Alessio, H. M. Exercise-induced oxidative stress. Medicine and Science in Sports and Exercise. Vol. 5. Num. 2. 1993. p. 218-224.

-AntunesNeto, J. M. F.; Pereira-da-Silva, L.; Macedo, D. V. Heat shock proteins as oxidative stress markers in rats submitted to exhaustive intermittent running training. Brazilian Journal of Biomotricity. Vol. 2. Num. 3. 2008a. p. 160-175.

-Antunes Neto, J. M. F.; Pereira-da-Silva, L.; Macedo, D. Heat shock proteins as oxidative stress markers in rats submitted to exhaustive continuous running training. Revista Brasileira de Educação Física, Esporte, Lazer e Dança. Vol. 3. Num. 4. 2008b. p. 116-130.

-Antunes Neto J. M. F.; Toyama, M. H.; Carneiro, E. M.; Boschero, A. C.; Pereira-da-Silva, L.; Macedo, D. V. Circulating leukocyte heat shock protein 70(hsp70) and oxidative stress markers in rats after a bout of exhaustive exercise. Stress. Vol. 9. Num. 2. 2006. p.107–115.

-Bienz, M.; Pelham, H. R. B. Mechanims of heat-shock gene activation in higher eukaryotes. Advances in Genetics. Vol. 24. 1987. p. 31-72.

-Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry. Vol. 72. 1976. p. 248-254.

-Craig, E. A. The heat shock response. Critical Reviews in Biochemistry. Vol. 18. 1985. p. 239-280.

-Craig, E. A. Chaperones: helpers along the pathways to protein folding. Science. Vol. 260. Num. 5116. 1993. p. 1902-1904.

-Didomenico, B. J.; Bugaisky, G. E.; Lindquist, S. The heat shock response is self-regulated at both the transcriptional and posttrancriptional levels. Cell. Vol. 31. Num. 3 (part 2). 1982.p. 593-603.

-Ellis, R. J.; Van Der Vies, S. M. Molecular chaperones. Annuals Reviews of Biochemistry. Vol. 60. 1991. p. 321-347.

-Essig, D. A.; Nosek, T. M. Muscle fatigue and induction of stress protein genes: a dual function of reactive oxygen species? Canadian Journal of Applied Physiology. Vol. 22. Num. 5. 1997. p. 409-428.

-Feige, U.; Polla, B. S. Heat shock proteins: the hsp70 family. Experimentia. Vol. 50. Num. 11-12. 1994. p. 979-986.

-Flaherty, K. M.; Deluca-Flaherty, C.; Mckay, D. B. Three-dimensional structure of the ATPase fragment of a 70Kda heat-shock cognate protein. Nature. Vol. 346. Num. 6285. 1990. p. 623-628.

-Gerner, E. W.; Scheneider, M. J. Induced thermal resistance in HeLa cells. Nature. Vol. 256. Num. 500-502. 1975. p. 500-502.

-Gething, M. J.; Sambrook, J. Protein folding in the cell. Nature. Vol. 355. Num. 6355. 1992. p. 33-45.

-Hall, T. J. Role of hsp70 in cytokine production. Experimentia. Vol. 50. Num. 11-12. 1994. p. 1048-1053.

-Halliwell, B.; Gutteridge, J. M. Free radicals in biology and medicine. Oxford: Clarendon Press, 1989.

-Hendrick, J. P.; Hartl, F. U. Molecular chaperone functions of heat-shock proteins. Annuals Reviews of Biochemistry. Vol. 62. 1993. p. 349-384.

-Laemli, U. K. Cleavage os structural proteins during the assembly of the head of bacteriophage T4. Nature. Vol. 227. Num. 5259. 1970. p. 680-685.

-Legendre, N.; Matsuidaira, P. Direct protein microsequence from imobilon-P transfer membrane. Biotechniques. Vol. 6. 1988. p. 1154-1159.

-Li, G. C.; Meyer, J. L.; Mak, J. Y. Heat-induced protection of mice against thermal death. Cancer Research. Vol. 43. Num. 12 (part 1). 1983. p. 5758-5760.

-Lindquist, S. The heat shock response. Annual Review of Biochemistry. Vol. 55. 1986. p. 1151-1191.

-Locke, M.; Noble, E. G.; Atkinson, B. G. Exercising mammals synthesize stress proteins. American Journal of Physiology. Vol. 258 (Cell Physiol. 27). 1990. p. C723-C729.

-Morimoto, R. I. Cells in stress: transcriptional activation of heatshock genes. Science. Vol. 259. Num. 5. 1993. p. 1409-1410.

-Neupert, W.; Hartl, F. U.; Craig, E. A. How do polypeptides cross the mitochondrial membranes? Cell. Vol. 63. Num. 3. 1990. p. 447-450.

-Rao, D. V.; Jones, G. L.; Watson, K. Comprehensive analysis of heat shock protein synthesis in human peripheral lymphocytes: the effect of penicillin/streptomycin. International Journal of Biochemistry and Cell Biology. Vol. 31. Num. 8. 1999. p. 861-868.

-Ritossa, F. A new puffing pattern induced by temperature shock and DNP in Drosophila. Experimentia Basel. Vol. 18. Num. 12. 1962. p. 571-573.

-Ryan, A. J.; Gisolfi, C. G.; Moseley, P. L. Synthesis of 70K stress protein by human leukocytes: effect of exercise in the heat. Journal of Apllied Physiology. Vol. 70. Num. 1. 1991. p. 466-471.

-Schlesinger, M. J. Heat shock proteins: the search for functions. Journal Cell Biology. Vol. 103. Num. 2. 1986. p. 321-325.

-Smolka, M. B.; Zoppi, C. C.; Alves, A. A.; Silveira, L. R.; Marangoni, S.; Pereira-da-Silva, L.; Novelo, J. C.; Macedo, D. V. HSP72 as a complementary protection against oxidative stress induced by exercise in the soleus muscle of rats. American Journal of Physiology. Vol. 279. Num 5. 2000. p. R1539-R1545.

-Sorger, P. K. Heat shock factor and the heat shock response. Cell. Vol. 65. 1991. p. 363-366.

-Storti, R. V.; Scotti, M. P.; Rich, A. Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell. Vol. 22. Num 3. 1980. p. 825-835,1980.

-Stuart. R. A.; Cyr, D. M.; Neupert, W. Hsp70 in mitochondrial biogenesis: from chaperoning nascent polypeptide chains to facilitation of protein degradation. Experimentia. Vol. 50. Num. 11-12. 1994. p. 1002-1011.

-Terlecky, S. R. Hsp70s and lysosomal proteolysis. Experimentia. Vol. 50. Num. 11-12. 1994. p. 1021-1025.

-Vigh, L.; Maresca, B.; Harwood, J. L. Does the membrane’s physical state control the expression of heat shock and other genes? Trends in Biochemical Sciences. Vol. 23. Num. 10. 1998. p. 369-374.

-Welch, W. J. Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiological Reviews. Vol. 72. Num. 4. 1992. p. 1063-1081.

-Welch, W. J. How cells respond to stress. Scientific American. Vol. 268. Num. 5. 1993. p. 34-41.

-Yellon, D. M.; Marber, M. S. Hsp70 in myocardial ischaemia. Experimentia. Vol. 50. Num. 11-12. 1994. p. 1075-1083.

Published
2012-07-18
How to Cite
Antunes Neto, J. F., Nader, B. B., & de Macedo, D. V. (2012). The standardizing experimental process to detect stress proteins in physical exercise. Brazilian Journal of Exercise Prescription and Physiology, 6(34). Retrieved from https://www.rbpfex.com.br/index.php/rbpfex/article/view/435
Issue
Vol. 6 No. 34 (2012)
Section
Scientific Articles - Original

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